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Entrance Microbiol. 2022 Might 3;13:836943. doi: 10.3389/fmicb.2022.836943. eCollection 2022.
ABSTRACT
Mt. Erebus, Antarctica, is the world’s southernmost lively volcano and is exclusive in its isolation from different main lively volcanic methods and its distinctive geothermal methods. Utilizing 16S rRNA gene amplicon sequencing and physicochemical analyses, we in contrast samples collected at two contrasting high-temperature (50°C-65°C) websites on Mt. Erebus: Tramway Ridge, a weather-protected excessive biomass web site, and Western Crater, an especially uncovered low biomass web site. Samples have been collected alongside three thermal gradients, one from Western Crater and two inside Tramway Ridge, which allowed an examination of the heterogeneity current at Tramway Ridge. We discovered distinct soil compositions between the 2 websites, and to a lesser extent inside Tramway Ridge, correlated with disparate microbial communities. Notably, pH, not temperature, confirmed the strongest correlation with these variations. The abundance profiles of a number of microbial teams have been totally different between the 2 websites; class Nitrososphaeria amplicon sequence variants (ASVs) dominated the neighborhood profiles at Tramway Ridge, whereas Acidobacteriotal ASVs have been solely discovered at Western Crater. A co-occurrence community, paired with physicochemical analyses, allowed for finer scale evaluation of parameters correlated with differential abundance profiles, with numerous parameters (whole carbon, whole nitrogen, soil moisture, soil conductivity, sulfur, phosphorous, and iron) exhibiting vital correlations. ASVs assigned to Chloroflexi courses Ktedonobacteria and Chloroflexia have been detected at each websites. Based mostly on the identified metabolic capabilities of beforehand studied members of those teams, we predict that chemolithotrophy is a typical technique on this system. These analyses spotlight the significance of conducting broader-scale metagenomics and cultivation efforts at Mt. Erebus to raised perceive this distinctive surroundings.
PMID:35591982 | PMC:PMC9111169 | DOI:10.3389/fmicb.2022.836943
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